A Critical Role for ATP in the Stimulation of Retinal Guanylyl Cyclase by Guanylyl Cyclase-activating Proteins
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چکیده
منابع مشابه
Inducing vasoconstriction by activating guanylyl cyclase.
OF THE MANY PHYSIOLOGICAL effects exerted by atrial natriuretic peptide (ANP), its direct influence on vascular tone seems to be the most puzzling. In the peripheral circulation, ANP binds to the extracellular ligand binding domain of the membrane-bound guanylyl cyclase isoform A (GC-A), which is widely expressed in vascular smooth muscle, to increase intracellular levels of cGMP (11). Activati...
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Retinal guanylyl cyclases (RetGCs) in vertebrate photoreceptors are regulated by the guanylyl cyclase activator proteins (GCAP1 and GCAP2). Here, we report EPR double electron-electron resonance (DEER) studies on the most ubiquitous GCAP isoform, GCAP1 and site-directed mutagenesis analysis to determine an atomic resolution structural model of a GCAP1 dimer. Nitroxide spin-label probes were int...
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Cyclic guanosine monophosphate (cGMP) is a critical second messenger that regulates cardiovascular function and vision in humans. Two recent papers, including one in BMC Structural Biology, have revealed atomic structures of the enzymes that catalyze the synthesis of cGMP providing new clues about the molecular basis of substrate specificity and allosteric regulation in nucleotide cyclases.
متن کاملNO activation of guanylyl cyclase.
Nitric oxide (NO)-sensitive guanylyl-cyclase (GC) is the most important receptor for the signaling molecule NO. Activation of the enzyme is brought about by binding of NO to the prosthetic heme group. By monitoring NO-binding and catalytic activity simultaneously, we show that NO activates GC only if the reaction products of the enzyme are present. NO-binding in the absence of the products did ...
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The GTP analog 5’-guanylyl-imidodiphosphate (Gpp(NH)p) caused marked stimulation of basal and catecholamine (isoproterenol)-sensitive adenylate cyclase from canine myocardium, frog erythrocytes and rat paraovarian fat. The combination of Gpp(NH)p (1OV M) and isoproterenol (10e4 M) produced activation of adenylate cyclase equal to (fat) or significantly greater than (heart, erythrocytes) that st...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2003
ISSN: 0021-9258
DOI: 10.1074/jbc.m303678200